描述
Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.
Features of TurboFect Transfection Reagent:
? High transfection efficiency of a wide variety of cell types
? No optimization required for most cell lines
? Excellent transfection efficiency in the presence or absence of serum
? Minimal cytotoxicity
? Ready-to-use—no need to reconstitute, dilute or manipulate
Applications of TurboFect Transfection Reagent :
? Stable and transient transfection or co-transfection of DNA or RNA
? Experiments with adherent or suspension cells, differentiated or undifferentiated cells
? Gene expression studies and assays involving GFP or luciferase reporters
TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.
規(guī)格
Cell Type: Endothelial Cells, Epithelial Cells, Established Cell Lines, Fibroblasts, Hepatocytes, Kidney, Macrophages, Myeloma Cells, Primary Cells
Sample Type (Specific): Plasmid DNA
Classification: Chemically Defined
Transfection Technique: Chemical Transfection, Lipid-Based Transfection
Product Line: TurboFect?
Product Size: 5 x 1 mL
內(nèi)容及儲(chǔ)存
Upon receipt store at 4°C.